The Effects of Cranberry Consumption on Lipid and Lipoprotein Metabolism in Human Apolipoprotein A-I Transgenic Mice Fed a High Fat and High Cholesterol Diet
Investigators: Christian Caceres, Dr. Ji-Young Lee, Dr. Young-Ki Park
The development of pathological conditions including cardiovascular disease are well documented to manifest from an obese state due to high lipid burden at adipose tissue and consequent low-grade inflammation. We hypothesized that anthocyanin-rich whole cranberry powder would prevent inflammation while simultaneously modifying high- density lipoprotein (HDL) metabolism to confer cardioprotection in C57BL/6J mice expressing human apolipoprotein A-I transgene (hApoAITg). Male hApoAITg C57BL/6J mice were fed a modified AIN-93M high fat/high cholesterol diet (HF/HC; 15% fat, 0.25% cholesterol by weight) with only the treatment group receiving 5% whole cranberry powder by weight for 8 weeks. Our results suggest that CR supplementation decreases obesity-induced inflammation in adipose tissue at least in part, by modulating energy metabolism in skeletal muscle. However, additional investigations are required to conclusively determine the effect of cranberry consumption on serum lipids and HDL metabolism.
Tyrosine Phosphorylation of the Bacterial Stress Factor BipA aids in Adaptation and Pathogenicity
Investigators: Akua Owusu and Jui Chaugule
Faculty Advisors: Dr Victoria Robinson and Dr David Benson
BipA is a multi-domain prokaryotic GTPase universally conserved in pathogenic bacteria. It regulates a number of virulence events including pedestal formation, flagella mediated motility and expression of virulence genes. Most importantly, BipA null mutants are avirulent, suggesting it is a prime target for antimicrobial development. Central to the function of BipA are its GTPase activity and its association with the ribosome. An examination of the ribosome binding properties of the protein revealed that BipA has two ribosome binding modes. Under normal growth conditions, GTP-bound BipA associates with 70S ribosomes. However, under conditions of stress, ppGpp-bound BipA associates with 30S ribosomes. A study by the O’Connor group at the University of Southampton (UK) demonstrated that BipA undergoes phosphorylation on one of its tyrosine residues and perhaps this modification may play a role in its ability to regulate virulence processes. Therefore, the purpose of my project was to identify the tyrosine phosphorylation sites in EHEC BipA and then determine how this modification affects its biochemical properties particularly its GTPase activity.
UConn Year of Graduation (Undergraduate): 2012 Undergraduate Major(s): Molecular and Cell Biology Currently Employed By: Waterbury Smiles and Old Greenwich Dental Associates, Licensed General Dentist Updates: Recently graduated from Danbury Hospital GPR and currently practicing comprehensive general dentistry in Waterbury and Greenwich, CT.
UConn Year of Graduation (Undergraduate): 2015 Undergraduate Major(s): Chemical Engineering Currently Employed By: Teach for America, Corps Member Updates: I am currently training to be a secondary math teacher through teach for america. I will be teaching in Hartford in the fall!
UConn Year of Graduation (Undergraduate): 2017 Undergraduate Major(s): Molecular and Cell Biology Currently Employed By: Dental Student Updates: I am starting dental school on August 9th! I am very excited, but also nervous.
UConn Year of Graduation (Undergraduate): 2014 Undergraduate Major(s): Nursing Currently Employed By: CT Children’s Medical Center, Clinical Nurse III Updates: I’ve recently passed a certification exam, earning the title of Certified Pediatric Nurse (CPN). Also, as of June 2017 I have passed my qualifying exams for the PhD program, earning a Master of Science in Nursing along the way.
UConn Year of Graduation (Undergraduate): 2016 Undergraduate Major(s): Molecular and Cell Biology Currently Employed By: Med Student Updates: Will be starting medical school at Albert Einstein College of Medicine this fall, class of 2021!
UConn Year of Graduation (Undergraduate): 2014 Undergraduate Major(s): Pathobiology Currently Employed By: , Updates: I am currently in my second year of medical school at UCONN. This past summer I completed research in Uganda.
UConn Year of Graduation (Undergraduate): 2013 Undergraduate Major(s): Individualized: Neuroscience and Ethics Currently Employed By: UCONN School of Medicine, Class of 2017 Updates: Currently in the process of matching into a Pediatric Residency and graduation in May!
UConn Year of Graduation (Undergraduate): 2015 Undergraduate Major(s): Biology, Chemistry Currently Employed By: Updates: Currently enrolled at UConn as a first year Dental student, class of 2020. Very excited!