Summer 2008: Optimizing Osteoblast Cell Culture for the Study of Dlx Gene Function

By Sean Ghassem-Zadeh

With the high volume of research involving in vitro studies and the need to optimize specific types of cell cultures, multiple companies have produced various Fetal Bovine Serum (FBS) products that can aid cell culture processes. With numerous companies producing multiple lots of FBS, which are obtained from an isolated population of fetal calves, a further investigation is needed to determine the best product for a desired cell culture (in this case calvaria and bone marrow cultures). By screening these different serums along with different lots of the same serum on both types of cell cultures, one can determine the most effective product. It is therefore proposed that a specific product list be determined and each serum be tested simultaneously with the same population of cells. In doing so, one can determine the best FBS for the two desired methods of cell culture.

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