Lab-on-a-chip Device for an Early Diagnosis of Cardiac Diseases
Investigators: Elena Carrington, Karim Abdel Jalil, Dr. Chandra Kumar Dixit in the Chemistry as well as the Molecular and Cell Biology Department
Through various experiments, we are showing that microfluidic arrays can be used for detection of cardiovascular disease. We are examining troponin, C-Reactive Protein (CRP), and myoglobin as biomarkers for detection of cardiovascular disease. These biomarkers are used in a 3D printed microfluidic device, which is designed with an open source designing software, Autodesk 123. The fabricated chip has two distinct regions, viz fluidics and detection zone. Reagent delivery system is constituted of five micro-channels for transporting sample and reagents to the detection chamber. Monoclonal capture antibodies are spotted separately within the detection chamber. The sample and reagents follow to the waste chamber. The detection zone is spotted with monoclonal antibodies specific to the three biomarkers. The objective of our experimental design is to develop a microfluidic-based tool for multiplexed and highly sensitive detection of cardiovascular diseases. The experiments are ongoing; therefore, we do not have conclusive results at this time.