Research

Rowe Researcher: Effects of mutants in the I-domain on Bacteriophage P22 coat protein stability and mature capsid structure

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Fejiro Okifo conducting research.
Fejiro Okifo conducting research.

February 2014-Present: Effects of mutants in the I-domain on Bacteriophage P22 coat protein stability and mature capsid structure

By Fejiro Okifo, Kevin Robbins, Dr. Carol Teschke

Department of Molecular and Cell Biology, University of Connecticut, Storrs

As common as the rhinovirus that visits itself upon us every flu season and as insidious as the HIV virus that causes AIDS, viruses have been afflicting the human race since the dawn of time. And yet there is still much to discover about the mechanism by which they infect their hosts. Continue reading →

Rowe Researcher: Measuring Viral Titer to Analyze Transduction Efficiency in Induced Pluripotent Stem Cells

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Spring 2011: Measuring Viral Titer to Analyze Transduction Efficiency in Induced Pluripotent Stem Cells

By Dr. X. Cindy Tian, Yong Tang, and Michael Tassavor

Stem cells have the ability to dramatically change the medical landscape, allowing regrowth and regeneration of tissues previously irreparable to modern science. Severe burns, spinal breakages, and muscle damage all can benefit, as well as more abstract diseases such as Parkinson’s, Multiple Sclerosis, and cancer. Until quite recently, however, sources for stem cells were limited to embryonic material.

In 2007, researchers managed to convert specialized adult cells to pluripotent stem cells capable of taking any somatic form in mice. This is highly useful in that it negates any need for embryonic stem cells in stem cell therapy, sidestepping legal and moral issues, as well as public outcry. Furthermore, it allows customized cells grown from the patient’s own cells, preventing immune rejection. Non-embryonic stem cells also have the benefit of allowing research to proceed with government grants, which, depending on the state and federal agencies involved, prohibit embryonic stem cell research. Continue reading →

Rowe Researcher: Investigating Propargyl-Linked Antifolates

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Summer 2012-Spring 2014: Investigating Propargyl-Linked Antifolates in Inhibiting Bacterial and Fungal Dihydrofolate Reductase

By Joshua Andrade, Dr. Amy Anderson’s Lab, and Dr. Dennis Wright’s Lab

Antimicrobial agents have been invaluable in reducing illness and death associated with bacterial infection. However, over time, bacteria have evolved resistance to all major drug classes as a result of selective pressure. The advancement of new drug compounds is therefore vital.  The Anderson-Wright Lab has focused on developing potent and selective inhibitors of dihydrofolate reductase (DHFR), an enzyme key in cell proliferation and survival, in several pathogenic species.  The lab has found that a set of compounds, known as propargyl-linked antifolates, are DHFR inhibitors that are both biologically effective and have strong pharmacokinetic properties. Continue reading →

Rowe Researcher: Assessing the 2012 NHANES Chemosensory Component

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Summer-Fall 2013: Assessing the Validity and Reliability of the 2012 NHANES Chemosensory Component

By Mallory Honda, Shristi Rawal, Dr. Valerie Duffy

In 2012, the National Health and Nutritional Examination Survey (NHANES) added a new chemosensory component to assess the prevalence of taste and smell disorders at a national level. NHANES is a nationally-representative survey of the U.S. population based on questionnaires and measures taken at mobile examination centers (MECs). The chemosensory component includes collection of self-reported data as well as taste and smell assessments carried out by researchers. Because the sense of taste is redundant (carried by many cranial nerves), reported loss of taste is rare and often actually due to decreased sense of smell which is much more liable to damage through aging, injury, or infection. Continue reading →

Rowe Researcher: Premenstrual Syndrome in Minority Women

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Fall 2012-Spring 2014: An Exploratory Pilot of Factors Associated with Premenstrual Syndrome in Minority Women

By Mallory Perry; Michelle Judge, PhD, RD; Deborah D. McDonald, PhD, RN

Research evidence is limited in relation to the difference between minority populations and White Americans in regards to premenstrual syndrome (PMS) symptoms.  Though no research has been done directly on PMS variances, studies on amount and duration of menstrual cycles do show that there is a significant difference between ethnic groups.  The aims for this research are to explore factors associated with PMS in minority women and to compare PMS symptom response of minority and nonminority women to diet supplementation with omega-3 fatty acids. Continue reading →

Lubonja’s research published in ‘Science of Advanced Materials’

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Before Klair Lubonja even started classes his freshman year he was engaged in research courtesy of the Pre-College Enrichment Program, sponsored by the Department of Health Career Opportunity Programs at the UConn Health Center. As a member of Dr. Yu Lei’s lab, Klair spent the summer working with copper nanowire and single-wired carbon nanotubes in an effort to enhance glucose electrooxidation. Continue reading →

Rowe Researcher: Translesional Synthesis DNA Polymerases

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Summer 2012: Structure and Interactions of Translesional Synthesis DNA Polymerases

By Maciej Kosakowski, Dr. Dmitry Korzhnev, Ph.D., Dr. Irena Bezsonova, Ph.D.

During my weeks with the College Summer Fellowship Program at the UConn Health Center, I worked in a structural biology lab in conjunction with the NMR lab under Dr. Korzhnev. I assisted him on his project, which aimed to discover the specific mechanisms behind translesional synthesis DNA polymerases, or TLS polymerases for short. Continue reading →

Rowe Researcher: Health in the Buduburam Refugee Camp

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Summer 2012: The Socio-Political Influences on Health in the Buduburam Refugee Camp

By Gian Grant, Dr. Elizabeth Holzer

The United Nations High Commissioner of Refugees (UNHCR) recognizes that there are approximately 2.7 million refugees in the world. The UNHCR often coordinates with non-governmental organizations and asylum countries to provide refugees with services such as health care until a permanent residential situation is agreed upon. Continue reading →

Rowe Researcher: Characterization of Drosophila Interacting Genes

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Daniel Camacho conducting research at the Health Center.
Daniel Camacho conducting research at the Health Center.

Summer 2012: Characterization of Drosophila Interacting Genes: Elucidating the Mechanism(s) of PolyQ Toxicity in Huntington’s Disease

By Daniel R. Camacho, Ping Zhang, Ph.D.

Polyglutamine expansions are a type of genetic mutation that is responsible for several human neurodegenerative diseases, including Huntington’s disease. The pathology of these diseases involves the accumulation of proteins containing polyglutamine domains within neuronal cells, which ultimately leads to cell death. The mechanism of toxicity of these protein aggregates is currently being investigated. My work involved using the model genetic organism Drosophila melanogaster to try to elucidate aspects of polyglutamine toxicity. Continue reading →

Rowe Researcher: Biofilm-Forming Bacteria

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Leonela Villegas conducting research.
Leonela Villegas conducting research.

Summer 2010: Determining the Effect of Spacing in Protection of Staphylococcus aureus by Pseudomonas Aeruginosa

By Leonela Villegas and Leslie Shor, Ph.D.

The research that I have been conducting since the summer of 2010 includes the observation of two different types of biofilm-forming bacteria: Staphylococcus aureus and Pseudomonas aeruginosa. Using a quad microfluidic device that simulates a controlled environment, I observed the interactions between these two bacterias at various distances from one another when being in contact with Tobramycin to ultimately compare these results to other types of antibiotics. Continue reading →